Phnshp45
Webb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 Webb7 jan. 2016 · The mcr-1-associated plasmid in the study by Liu and colleagues, pHNSHP45, is an approximate 64 kb IncI plasmid with substantial sequence homology (≥80% sequence identity over ≥80% of the query) to only four other plasmid sequences in GenBank (Accession numbers: JN983044.1, KJ460501.1, KM202412.2, CP009581.1).Using …
Phnshp45
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Webb6 feb. 2024 · Except for a 3,684 bp region that was also present in a pHNSHP45-2-like plasmid and included the ISApl1 and mcr-1, the entire nucleotide sequence showed >99.9% identity to the corresponding region ... Webb1 jan. 2016 · pHNSHP45 to the recipient strain. pHNSHP45 could be transferred via transformation, but not conjugation, into K pneumoniae 1202 (ST11) producing KPC-2, E coli E11
Webb15 mars 2024 · The pHNSHP45 plasmid-encoded PcnR protein favours the fitness of bacteria carrying mcr-1-bearing IncI2 plasmids. (A) PcnR enhances the fitness of E. coli … Webb24 maj 2016 · In particular, the pHNSHP45 3 is the only one mcr-1-carring plasmid with known genome sequence prior to our study. Unlike the other mcr-1 -positive plasmid pKH457-3-BE 2 that is from the cattle isolate in Belgium and has the plasmid signature of IncP, both pHNSHP45 3 and pWH12 are hosted by E. coli isolated from swine in China, …
WebbThe PCR based mapping of mcr-1 genetic context in our isolates revealed that almost all amplified genes were similar to pHNSHP45, except the truncated tnpA gene, missing IsApl1. Globally, plasmid analysis of various Incompatibility types has shown that IsApl1 is usually absent in IncX4 type plasmid while in Incl2 type plasmid, it is either absent or … Webb10 jan. 2024 · Construction of the Deletion Mutant. The coding region of the mcr-1 gene was deleted from E. coli K12 BW25113 carrying plasmid pHNSHP45 (Liu et al., 2016) following a one-step inactivation method (Datsenko and Wanner, 2000).The primers used in this study are shown in Supplementary Table S1.PCR products containing …
WebbPlasmid Transformation Assays. The competent cells including E. coli DH5α containing pUC19-mcr-1 and E. coli C600+pHNSHP45 were prepared and used for transformation assay following the protocol. 14 Mixing 100 μL of chemically competent cells with 10 μL of plasmid pCas9-m1 or pCas9-m2 incubated the reaction mixture on ice for 30 min, and …
WebbSequence alignment of seven IncI2-type mcr-1-bearing plasmids. pHNSHP45 with GenBank no. KP347127 was used as a reference to compare with other mcr-1-bearing plasmids which possess the IncI2 replicon. イオン高砂 paypayWebb1 feb. 2024 · Introduction. Sir Alexander Fleming may have hardly anticipated that his serendipitous discovery of penicillin used to control bacterial diseases, which heavily … イオン高砂チラシWebb18 nov. 2015 · To identify the gene responsible for polymyxin resistance, pHNSHP45 was completely sequenced, which revealed a plasmid 64 015 bp in size with an average GC content of 43·0%. pHNSHP45 contains 60 predicted open reading frames and possesses a typical IncI2-type backbone (57 624 bp) encoding replication, horizontal transfer, … イオン高砂事故Webb21 okt. 2024 · The pHNSHP45 possesses a typical IncI2-type backbone encoding replication, horizontal transfer, maintenance, and stability functions. Citation 1 The … otto grotewohl straßeWebb19 dec. 2016 · Compared with the original mcr-1-bearing IncHI2 plasmid pHNSHP45-2, 23 three regions in which antibiotic resistance genes were observable were found to exhibit structural differences (Figure S2). First, the IS Apl1 - mcr-1 - orf region located between terF and the hypothetical CDS was absent in pHYEC7-IncHI2, indicating that mcr-1 could … otto grotewohl spdWebb17 jan. 2024 · IncHI2 plasmids, such as pHNSHP45–2, have been frequently associated with global spread of mcr-1 genes (3,13,14). Figure 1 Sequence comparison of scaffolds … otto grotewohl stadionWebb图4 大肠杆菌 SHP45 携带 mcr-1 基因质粒 pHNSHP45 的结构. 3. 多黏菌素耐药现状. 由于质粒介导的 mcr-1 基因可以在不同菌株间进行水平传播，致使动物源性多黏菌素类药物耐 … イオン高知