Nettet16. mar. 2024 · Mouse macrophages exhibit organ-specific differences in expression of macrophage markers. Hepatic macrophages are labeled for CSF1R, Mac2 and F4/80, … NettetStreptococcus pneumoniae is the most prevalent cause of community-acquired pneumonia and is known to induce apoptosis and necrosis in macrophages in vivo. We analyzed …
Development of an Optimized Culture System for Generating Mouse ...
Mouse AMs from bronchoalveolar lavage (BAL) can be cultured20 and expanded in granulocyte-macrophage colony-stimulating factor (GM-CSF)-containing medium19. We observed that these cells, hereafter referred to as exAMs, could be kept in continuous culture for at least 10 months (Fig. 1a), resulting … Se mer The microenvironment is a potent determinant of macrophages2,5. Because the transfer from the in vivo environment to ex vivo culture results in major gene expression changes in … Se mer We next investigated the epigenetic mechanisms underlying the full restoration of transcriptomic AM identity and loss of the transcriptional culture adaptations of texAMs and used ATAC-seq analysis to identify potential … Se mer To determine whether the transcriptional adaptations to the culture environment were stable or reversible, we used intratracheal transplantations to establish whether the original transcriptional identity of AMs could be … Se mer To test whether exAMs could reconstitute an empty AM niche, we used Csf2rb−/− mice, which lack the β chain of the GM-CSF receptor, are deficient in AMs and can be reconstituted … Se mer NettetI typically see 2-3E5 alveolar macs from one mouse. I usually pool cells from multiple mice to get enough for an experiment. I have had luck with using 0.5mM EDTA/PBS for … barber 92807
Inflammation-Induced Alternative Pre-mRNA Splicing in Mouse …
NettetThese processes involve tissue-resident alveolar macrophages (AMΦ) as well as recruited bone marrow (BM) ... (PMSCs) reduce the population and function of M1 bone marrow MΦs (BMMΦs) while preserving M2 alveolar MΦs (AMΦ) population in mice with Klebsiella pneumoniae (KP) pneumonia. (A, pooled data; B, representative data) ... Nettet24. sep. 2024 · Alveolar macrophages (AMs), including tissue-resident alveolar macrophages (TR-AMs) and monocyte-derived alveolar ... Schepper S, Chakarov S, … Nettet13. apr. 2024 · This study explored the effects of A. stricta root extract (AsE) in ovalbumin (OVA)-induced allergic asthma and lipopolysaccharide (LPS)-stimulated macrophages. Administration of 100–400 mg/kg AsE dose-dependently decreased pulmonary congestion and suppressed the reduction of alveolar surface area in mice with OVA-mediated … barber 92672