Bt2a bt2b primers
WebOct 28, 2024 · For β-tubulin gene, TU1/TU2 primers was performed by initial denaturation at 95 °C for 4 min, followed by 35 cycles of 95 °C for 30 s, 55 °C for 30 s, 72 °C for 1 … WebBt2a/Bt2b primers were used to amplify the beta-tubulin gene of NJA-1. Sequence analysis the PCR product and morphology observation indicated that NJA-1 belonged to …
Bt2a bt2b primers
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WebIsolation and identification of pathogen from rose plant was done by observing phenotypic characters which were further executed on molecular bases using ITS, EF and Bt2a/Bt2b primers. On these bases, pathogen was identified as Fusarium incarnatum. WebFeb 1, 2024 · The segment of the β-tubulin gene of the fungal DNA was amplified using primers bT2a and bT2b (Glass and Donaldson, 1995). Similarly, a region of the calmodulin gene of the fungal DNA was also amplified using the primers cmd5 and cmd6 as described by Hong et al. (2006 ).
WebAmomum villosum is widely planted as a cash crop and medicinal plant. Recent reports of leaf diseases that result in yield reduction of Amomum villosum fruits raise concerns, which may play an important role in yield losses. Understanding the aetiology of current and new diseases of Amomum villosum is important to address production issues and to … WebJul 25, 2024 · For molecular identification, DNA was extracted and the internal transcribed spacer (ITS) region of ribosomal DNA and portions of the β-tubulin and elongation factor 1 alpha (EF1-α) genes of a selected isolate (Gc_01) were amplified using primers ITS1/ITS4 (White et al., 1990), Bt2a/Bt2b (Glass & Donaldson, 1995) and EF1-728F/EF1-1199R …
WebAug 16, 2024 · For molecular identification the internal transcribed spacer of rDNA region (ITS), the genes of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT) and β-tubulin (TUB), and Apn2-Mat1-2 intergenic spacer (ApMat) were sequenced using ITS1/ITS4, GDF/GDR, ACT-512F/ACT-783R, Bt2a/Bt2b, CgDL-F6/CgMAT1-F2 … WebThe electrophoretogram showing the behaviour of primer pair Bt2a and Bt2b in a temperature gradient (annealing 50–60 °C). The specificity for the tubC paralogue …
WebSep 13, 2024 · The internal transcribed spacer (ITS) and β-tubulin gene (TUB) regions were amplified from DNA of the representative isolate WNp31 with primers ITS4/ITS5 and Bt2a/Bt2b (Glass and Donaldson 1995 ).
WebNucleic acid sequence analysis is an extensively applied method in Genomics studies. Universal primers are complementary to nucleotide sequences that are very common in a particular set of DNA molecules and cloning vectors. Thus, they are able to bind to a wide variety of DNA templates. During sequencing, primers are annealed to the denatured ... shop houses costWebMA). The primers were Bt2a (forward): 5¢-GGTAACCAAATCGG TGCTGCTTTC-3¢, and Bt2b (reverse): 5¢-ACCCTCAGTGTAGT GACCCTTGGC … shop houses for sale in texasWebMay 16, 2024 · The ITS, EF1-α and tub2 gene regions of the reference isolate (Code: WL2-D) were sequenced using primers ITS1/ITS4, EF1-728F/EF1-986R and BT2a/BT2b, and the sequences were deposited in GenBank with accession numbers MZ028464 (ITS), MW862012 (TEF1-α) and MZ028463 (tub2). shop houses for sale arkansasWebPrimer sequence: Primer concentration: Amplification of long PCR products Optimizing cycling conditions Optimizing PCR additives Optimizing 3' to 5' exonuclease activity Enzymes used in PCR Taq DNA polymerase Hot-start DNA polymerase High-fidelity DNA polymerase PCR cycling Commonly used terms in PCR PCR quantification shop houses for sale singaporeWebapplied. The amplicons of β-tubulin gene (primers Bt2a/Bt2b) were digested with AlwI (BspPI) enzyme (Thermo Scientifi c, USA) for 4 h at 55 °C and heat-terminated at 80 °C … shop houses for rent in singaporeWebMar 23, 2013 · Partial sequences of the beta-tubulin gene were determined by using the primers Bt2a and Bt2b for PCR amplification and sequencing and underwent a BLAST search at the National Centre for Biotechnology Information, these results confirmed that the isolated fungus was P. digitatum. Conclusion shop houses for sale in missouriWebSep 1, 2024 · Bt2 primers can amplify either benA fragments (three introns) or paralogous tubC fragments (two introns) in the Nigri section. When a mix of those two paralogues is used in phylogenies, this can create incongruent … shop houses for sale