WebApr 2, 2024 · We constructed cell mutant models, including NLS-YAP15SA (nuclear localized), YAP1S94A (incapable of binding to the TEA domain transcription factor … WebJun 14, 2024 · For histological assessment, the isolated cardia of the gastric tissue was fixed in 4% formaldehyde (BL539A, Biosharp, Anhui, China) (Lin et al. 2024). Then, tissue samples were embedded in paraffin. Paraffin sections were subsequently sliced into thin slices with a thickness of 4 μm. The tissues were dewaxed and subsequently stained with ...
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WebTissues were fixed with 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China) and embedded in paraffin. Next, each section (thickness, 5-lm) was deparaffinized, rehydrated, sub-jected to antigen retrieval, and submerged in 3% hydrogen perox-ide (10 min). Then, the slides were flushed with Tris-buffered WebSep 9, 2024 · Township of Fawn Creek in Montgomery County, KS. They specify cooking areas are the heart of the home – nonetheless they’re also the heart of the home’s … bishop hoffman catholic schools employment
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WebMar 20, 2024 · Abstract Here, we present a protocol for the detection of the two STING isoforms (erSTING and pmSTING) in human peripheral blood mononuclear cells or mouse splenocytes using Western blot and PCR. We detail steps to construct plasmids encoding each isoform and transfer them into mouse and human cell lines. Webprovides a secure, easy-to-use graphical interface for configuring the module via HTTPS. SNMPv1, v2c, and v3. facilitates management of the switch, as the device can be … WebDec 3, 2024 · PBS and 4% paraformaldehyde (PFA) (#BL539A, biosharp, China). 2.2 Equipment. Injection syringe: 10 μL microinjection syringes (0.7 mm tip diameter) were … dark magician deck with slifer